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Where DNA-barcoding proved inconclusive, algae were subjected to morphological examination at the light microscope level and images compared to online culture collections (e.g. the Culture Collection of Autotrophic Organisms (CCALA)).
Based on morphological examination, at least 55 Tuber samples were selected for molecular analyses (excluding sequences from Genbank database).
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This indicates the need of a morphological examination of at least 2 ejaculates (not the first one collected) for evaluation of the morphological quality of the semen.
It has been suggested that as a rule, classification of cell death in a given model should always include morphological examination coupled with at least one other assay [ 26].
Morphological examination of MCHODN nanoparticles at N/ P ratio 3 : 1 was investigated by transmission electron microscopy (TEM, JEM 1200, EX 11, JEOL, Japan).
The gonads were weighed, subjected to morphological examination and then either stored at −70°C prior to E2 and 11-KT measurement or immediately fixed in 4% formalin solution for histology.
Morphological examination of the embryos revealed that at E7.5 and E8.5, the Grp94−/− embryos were much reduced in size with no recognizable structure, compared to normally developing Grp94+/+ and +/− embryos (Figure 4B).
For morphological examination and comparison of the different TS models, TSQ at the end of the growth study (i.e. day 25) and TSP of equivalent size (approximately day 8) were used.
The kidneys were either embedded in epon for morphological examination or snap-frozen in liquid nitrogen and then stored at -70°C until further study.
The morphological examination of the chitosan nanoparticles was performed by transmission electron microscopy at an accelerating voltage of 100 kV (TEM, JEM 1200CX II, JEOL).
Individual Chelex samples containing the exoskeleton of extracted thrips have been deposited in a −20°C molecular specimen freezer at the University of Queensland and are available for morphological examination upon request.
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