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To quantify the degree of morphological disruption in eye epithelia, confocal stacks were imported into Image J (NIH), 3D reconstructions were made using Volume Viewer, and images were thresholded to gain area measurements of deep ElaV cells.
Furthermore, we showed that the EPIYA motifs of CagA are necessary for proper apical targeting in the polarized retinal epithelial cells, and loss of these motifs renders CagA less potent in inducing morphological disruption in the epithelium.
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Hematoxylin and eosin staining showed no morphological disruption of the retina in the in vivo study.
Administration of drugs by intravitreal injection can result in morphological disruption of the retinal structure, caused by the injection procedure and tissue inflammation.
In the present study, the mitochondria in F20 group showed morphological disruption whereby the sizes were increased, the cristae were disrupted, and the matrixes were hypodense compared to C group.
We showed that the reduced potency of the CagAEPISA mutant could not be explained by a role for SHP-2/Csw in the morphological disruption.
Although the growth plates of MEPE-overexpressing mice display severe morphological disruption, the expression and function of MEPE in growth plate matrix mineralization remains largely undefined.
Because GMR-Gal4 drives expression initially in third instar larvae, we assessed morphological disruption at this stage.
To assess the degree of morphological disruption, we examined the pattern of actin and ElaV expression in each eye epithelium.
It was reported that morphological disruption of myofibrils was associated with the magnitude of decrease in muscle function (Raastad et al. 2010).
Our work in the eye epithelium argued that CagA induces morphological disruption by activating the Rho/MLC pathway.
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