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Despite the geographical barrier and clear morphological differentiation between the populations of the two regions, recent genetic analyses suggest maintenance of genetic flow.
Moreover pre-mating isolation may be more effective at maintaining morphological differentiation between divergent populations.
The morphological differentiation between S-RED and BLUE could be a result of sexual selection, with the potential proximal visual cues for assortative preference in BLUE females being male head width, as well as potentially throat colour.
Combined with previous estimates of lineage-wide gene flow, these results are consistent with the suggestion that assortative female preference is more effective than post-mating isolation in maintaining morphological differentiation between divergent populations.
To determine whether loss of morphological differentiation between rainforest and ecotone habitats was greater in West than in Central Africa, we analyzed variation in PC 1 and log-transformed morphological traits between rainforest and ecotone.
There also appears to be a size component to behavioral and morphological differentiation: larger animals were found to be less selective than smaller taxa, although at body sizes below about 100 kg, morphological differentiation between snouts seems to be minor [40].
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To test for a consistent eco-morphological differentiation between color morphs across the species complex, logistic regression with a binominal error distribution was used and conducted in R v. 2.15.1 [ 55].
Galen and collaborators were among the first to show local variation in phenotypic selection apparently generating pollination ecotypes; divergent selection was associated with genetic and floral-morphological differentiation between Polemonium (Polemoniaceae) populations in the alpine and subalpine (Galen and Newport 1988; Galen 1989; Galen et al. 1987 , 1991.
A similar strategy is applied to describe morphological differentiation patterns between individuals of a L. dufourii population from Eastern Spain [ 54].
Similarly, previous studies indicated that most of the protein expression and morphological differentiation observed between the ecotypes in the same population were not plastic [ 66, 67].
The morphological differentiation observed between these populations of a well described subspecies (B. j. calurus) indicates that variation in morphology alone may not accurately reflect speciation events.
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