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Vertical scanning interferometry (VSI) is a novel technique that allows for the expedient morphological determination of single cells.
The morphological determination of the specimens in this study is consistent with their assignment to one of the two populations.
Molecular analysis fully confirmed morphological determination of species identity for the 16 analysed regenerated specimens of L. pulmonaria and the 6 of P. adscendens.
Morphological determination of levels of apoptosis was performed by labelling the cells with the nuclear stain Hoechst 33258 and visualisation by fluorescence microscopy.
* origin concluded from morphological determination of captive individuals.
Light and electron microscopic studies were used for the morphological determination of the fungal species.
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pipiens complex populations in California; and 5) determine whether morphological determinations of autogeny (or anautogeny) are consistent with genetic cluster assignments.
Importantly, in all of these investigations, they emphasized the combination of fluorescence staining and morphological determination with LSC as being of utmost importance for the identification of apoptotic cells.
Unlike the calcium images taken in a single plane, images of the Alexa Fluor 647 filled motor neuron were acquired before and after the end of the experiment using 50 70 1 µm steps (Mipos 100SG piezo driver; Piezosystems Jena, Jena, Germany) to create stacks for the purpose of full morphological determination.
Morphological determination was based on morphometry/morphology of the shell and reproduction organs such as bursa copulatrix and its duct [ 15, 29].
By distinguishing mutant and normal alleles, this technique allows both quantitative analysis of the ribonuclear inclusions of repeat expansions and their morphological determination.
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