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In northern Brazil, the current diagnostic procedures at the hospital laboratories for the detection and identification of invasive fungal infections include culture (blood cultures, clinical specimens cultured on selective fungal media), biochemical methods, microscopic morphological determination and immunological assays.
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Importantly, in all of these investigations, they emphasized the combination of fluorescence staining and morphological determination with LSC as being of utmost importance for the identification of apoptotic cells.
By distinguishing mutant and normal alleles, this technique allows both quantitative analysis of the ribonuclear inclusions of repeat expansions and their morphological determination.
Morphological determination was based on morphometry/morphology of the shell and reproduction organs such as bursa copulatrix and its duct [ 15, 29].
Genes involved in growth of mature Acropora colonies include the classical signaling pathways associated with development of cnidarian larvae and polyps as well as morphological determination in higher metazoans.
To explore the molecular mechanisms underlying the morphological determination of the ALT phenotype, we analysed expression of hTR and hTERT at the RNA level.
Light and electron microscopic studies were used for the morphological determination of the fungal species.
Molecular analysis fully confirmed morphological determination of species identity for the 16 analysed regenerated specimens of L. pulmonaria and the 6 of P. adscendens.
Vertical scanning interferometry (VSI) is a novel technique that allows for the expedient morphological determination of single cells.
The morphological determination of the specimens in this study is consistent with their assignment to one of the two populations.
Morphological determination of levels of apoptosis was performed by labelling the cells with the nuclear stain Hoechst 33258 and visualisation by fluorescence microscopy.
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