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A wide range of morphological defects was observed in the 12 lines.
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HRTEM images show that significant morphological defects are present on the surface of MgO microcrystals in both cases.
The observed morphological defects are accompanied by disturbances in the expression and localization of previously identified and novel molecules related to cell adhesion/interaction and cytoskeletal dynamics.
Although we cannot rule out that there are some subtle muscle defects at extrasynaptic sites, our data illustrate that synaptic electrophysiological and morphological defects are already present in these mild loss of function conditions.
For each of these 18 plasmids, we verified that suppression was linked to the plasmid, the plating efficiencies when rseA and rseB were overexpressed were no longer reduced, and the morphological defects were significantly reduced.
No evidence of outer membrane vesiculation, which has been associated with alterations in σE activity [33], [34], or other gross morphological defects were observed in the TEM or SEM images.
These morphological defects are mirrored by the progressive reduction of the territories of Dlx gene expression which is first reduced only in proximal parts of the 1st PA and then progressively lost from most of the 1st PA, persisting always only in the distalmost region.
These morphological defects were alleviated in the presence of an osmotic stabilizer suggesting that absence of septins affected the proper formation of the cell wall, which was coherent with a higher sensitivity of septin defective cells to drugs that affect cell wall construction as well as exocytosis.
Dosages were then decreased until concentrations that did not induce morphological defects were defined.
Embryos with no fluorescence or with gross vascular morphological defects were excluded from quantification.
In all experiments, data from fish with morphological defects were not included in statistical analyses.
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CEO of Professional Science Editing for Scientists @ prosciediting.com