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Flies homozygous mutant for any of the four Sans alleles were adult viable, male and female fertile, and had no gross morphological defects (data not shown), indicating that Sans is not essential for fly development and fertility.
Although the axonal MT cytoskeleton had no obvious morphological defects (data not shown), we observed abnormalities in the distribution of proteins and synaptic vesicles along the nerves.
Of interest, keyholes were seen in some embryos that lacked other obvious morphological defects (data not shown), suggesting that the region of the pharyngeal attachment is particularly sensitive to deformation after partial degradation of the sheath.
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These constructs were targeted normally to the Golgi apparatus, and the cell lines showed no morphological or proliferative defects (data not shown).
We found that Utx /Utx homozygotes indeed only rarely survive to adults (Fig. 1C) but that Utx /Df 2L BSC143 transheterozygotes develop into adults at a frequency comparable to Utx Δ/Df 2L BSC143 transheterozygotes or Utx Δ homozygotes and that these animals show no detectable morphological defects (Fig. 1C; data not shown).
In treated embryos, progression to the blastocyst stage was slowed, but we detected no increase in gross morphological defects (Fig. 1A and data not shown).
In all experiments, data from fish with morphological defects were not included in statistical analyses.
The results showed good crystalline behaviors and morphologies without any identifiable morphological defects.
Although we cannot rule out that there are some subtle muscle defects at extrasynaptic sites, our data illustrate that synaptic electrophysiological and morphological defects are already present in these mild loss of function conditions.
On their own, these suppressor alleles displayed wild-type seam cell number and alignment and had no obvious morphological defects (Table I, see groups B and C; and data not shown).
Ducts within Int6sh-expressing mammary glands, that did not develop tumors, showed multiple morphological defects including decreased secondary branching and termination at blood vessels (data not shown).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com