Other than a moderate increase in mortality, there were no consistent morphological defects observed at 24 hpf.
Additionally, him-8 e1489); him-8 e1489 animals display grol-3 e754ological rol-3 e754 manimalscific structures, consistent with improperly formedisplaycumulated cuticle (Supportingrossormorphological1c–defects
Our anther transcriptome data provided spatially more specific information for the expression patterns of metabolism-related genes (Supplemental figure 4 in Additional file 1) and showed that the expression levels of genes involved in cell wall formation, lipid synthesis and secondary metabolism were obviously altered in the ams anther, consistent with morphological defects.
Although most clutches of uninjected or control-injected larvae had a few larvae that displayed a range of phenotypes that deviated from the appearance of healthy larvae, which we labeled as 'normal', the morphological defects in mpi morphants were specific, consistent and relatively homogeneous within two distinct groups, which we labeled as 'mild' or 'severe' phenotypes.
Furthermore, the morphological defects of stereocilia in Pls1 KO are consistent with an abnormal maintenance of their actin content.
Because the morphological defects observed in Atmin Gpg6/Gpg6 kidneys were consistent with perturbation of the Wnt/PCP pathway, expression of genes relevant to canonical Wnt and non-canonical Wnt/PCP signaling were examined.
The results showed good crystalline behaviors and morphologies without any identifiable morphological defects.
Closer examination of these arrested animals revealed the presence of subtle morphological defects and a distinctive anterior constriction of the cuticle, consistent with the retention of an incompletely shed cuticle (Fig. 3f j and Table 1).
These findings were also consistent with the lack of gross morphological defects seen in fbn-1 single mutant embryos as well as mec-8; sym-3 or mec-8; sym-4 double mutants, which nevertheless had a prominent keyhole and Pin phenotype.
The pharyngeal defects are consistent with a recent report that demonstrated morphological defects of the lower jaw in both s1pr2 and spns2 mutants (Balczerski et al., 2012).
