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Second, although the secreted sulfatase-deficient skeletons appeared overall to be patterned normally (Fig. 4A and B), we did observe several highly specific morphological defects at the midline, including sternal fusion and defective basisphenoid patterning.
F-actin and DAPI staining did not reveal morphological defects at any stages of oogenesis in M601 (Fig. S2; data not shown).
Recently, Wang et al. reported that a TG-RNAi strain with reduced expression of TG using an ACt5C-GAL4 driver showed no morphological defects at 22°C [16].
Interestingly, control and DKO littermates showed no noticeable morphological defects at E8.5 (8–12 somite stage) and were histologically indistinguishable.
BSA-bead implanted embryos develop normally and have no morphological defects at 24 hpf, similar to control embryos.
We failed to detect morphological defects at the attachment site of the intestine to the rectum in any of the mutant backgrounds examined.
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An evaluation of the cell morphology at 30°C and 37°C growth temperatures established KN99α rom2 has a morphological defect at 37°C growth conditions (Figure 1A and B).
Depletion of Notch in trachea led to a morphological defect at the proximal DB.
Depletion of Notch in trachea caused a morphological defect at the proximal DB.
Other than a moderate increase in mortality, there were no consistent morphological defects observed at 24 hpf.
However, morphological defects are observed at the anterior end of the telencephalon.
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