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Labelling of cells was shown to be correlated with typical morphological criteria of apoptosis.
For studies 2,4,5,6,7 & 8 the definition of a NGF is according to the morphological criteria of Block and Gougeon [8], [11]: an NGF was counted when a clearly defined oocyte nucleolus was present within the optical dissector counting frame.
Amoebae were preliminarily identified to the species level using the morphological criteria of [ 20].
Nonlabelled cells that satisfied morphological criteria of apoptosis were counted as apoptotic cells.
Pericentric regions of all chromosomes matched these morphological criteria of heterochromatin.
Embryos were harvested at various days throughout development and staged according to the morphological criteria of Hamburger and Hamilton [ 15].
Similar(41)
In more recent times, cladistics have come to the fore and, with the advent of molecular biology, started to increasingly supplant the use of morphological criteria for discrimination of phenotypically similar species or species of uncertain ancestry.
Zona pellucida (ZP), which is a filamentous matrix of well-structured and glycosylated glycoproteins surrounding the oocyte, is another morphological criterion of oocyte selection.
The TCNS was selected as the criterion standard for DSP in this study in the absence of a single, universally accepted gold standard for DSP and the prior demonstration of validity of the TCNS against the morphological criterion of sural nerve fibre density [ 10].
As morphological criteria for identification of "giant" boutons we defined proximity to thorny excrescences of CA3 pyramidal neurons, their size and at least 1 filopodium.
Morphological evaluation of CK-positive cells is thus crucial for the correct diagnosis of DTCs, and in the present study, we applied the morphological criteria for diagnosis of DTCs analysed by IF and IC [ 8, 18].
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