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Principal components analysis (PCA) was performed on 10 morphological characters (excluding the meristic character) and a varimax rotation [ 61] was applied to all components that had Eigenvalues greater than 1.
Similar(59)
Species/subspecies of the species complex shared most morphological characters, except for lip-midlobes and calli.
This final alignment yielded RC values of 0.3878 (indel characters included) and 0.3763 (indel characters excluded).
Ranges of ambiguously aligned characters excluded prior to analyses.
To test the monophyly of Broussonetia sensu Corner (1962), Clement and Weiblen (2009)'s aligned DNA matrix of chloroplast ndhF and nuclear 26S (TreeBASE Study ID S2229) assembled for phylogenetic analyses of Moraceae was adopted, with morphological characters of the matrix excluded.
Probable reasons for this are that in immature stages there appears to be no morphological characterization that would distinguish Ditrysia from monotrysians, and the methodological decision to exclude morphological characters only applicable for resolving interrelationships within Monotrysia.
To investigate the effect of including functionally convergent characters, we reanalyzed the morphological data excluding characters that both: (i) unambiguously supported spurious clades, and (ii) were related to feeding ecology (i.e. concerning dentition, tongue structure and musculature); results are shown in Table 4.
To address potential issues of character exclusion [ 58], we explored the effect of excluding those morphological characters suspected to be functionally coupled with social parasitism by repeating the reconstruction when including them.
Results of comparisons of phylogenies obtained excluding potentially convergent morphological characters and COX1 third codon positions, and down-weighting (MP and ML) or excluding (Bayesian) CYTB third codon positions and mtrDNA loops are shown in Table 6.
Pursuing our second aim, we excluded from the set presented by [ 38] the three behavioural/ecological characters (among others the occurrence of social parasitism), and on the basis of information from [ 1, 2, 13, 54, 55, 63, 64] we further excluded 37 morphological characters, including four of the new ones.
Assuming that all or some of these 18 reductive or simplified morphological characters likely represent homoplasy, we excluded them from the original dataset and the reduced dataset was subjected to maximum parsimony (MP) analysis, similar to that conducted by Pietsch and Orr [ 33].
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