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Analysis of the morphological changes was done by using scanning electron microscopy (SEM) and atomic force microscopy (AFM).
It was found that the use of light microscopy in the identification of cell morphological changes was a valuable adjunct in verifying the results of colorimetric tests.
Observation of morphological changes was conducted under ambient atmosphere at room temperature (22 24 °C) using an Olympus IX70 inverted microscope equipped with phase contrast optics.
A sequential increase in morphological changes was observed during the time course.
Forty-eight hours (h) after splitting, hES cells were treated with BMP-2, BMP-6 or BMP-2/6 at 100 ng/ml in mTeSR1 for 5 days, and the time course of morphological changes was analyzed.
An effect of IFIT4 on monocyte differentiation into DCs in terms of morphological changes was observed.
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In both cases, considerable morphological changes were not observed.
Morphological changes were further evidenced in the higher number of GFP-expressing GPCs (Supplemental Fig. 2B).
(a) The morphological changes were imaged using phase contrast microscopy (shown at 200x magnification).
Fast morphological changes are described in astrocytes in response to increased intracellular calcium45.
The surface morphological changes were characterized using SEM.
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