We now briefly review this literature, starting with visible morphological changes and then turning to matters of behavior and neurobiology.
To investigate the effects of L-DOPA on cortical neurons, the cells were treated with different concentrations of L-DOPA (0, 1, 5, 10, 100 or 200 µM) in DMEM+5%FBS for 16 hours and then morphological changes, neuronal viability, cell iron content, the expression of DMT1−IRE, DMT1+IRE, TfR1 and Fpn1 proteins and NTBI (non-transferrin bound iron) uptake were determined.
Morphological changes of the cells were then observed under a inverted light microscope (Olympus CKX41SF, Japan).
We then observed morphological changes in these neurons for 12 h using time-lapse imaging.
Then the morphological changes in the Hoechst 33342-stained nuclei of cells were observed using a fluorescence microscope (Leica, Wetzlar, Germany).
A549 cells were incubated with different concentrations of saffron extract; then cell morphological changes, cell viability, and apoptosis were determined by the normal invertmicroscope, MTT assay, Annexin V and propidium iodide, and flow cytometric analysis, respectively.
These clusters follow the inverse Ostwald ripening whereas if e-irradiation is performed at RT then only the morphological changes in both carbon nanotube as well as nanorod are observed.
Each patient-derived cell line was then assessed for morphological changes by electron microscopy (EM).
We then verified the morphological changes of PC3 cells treated with the agents alone or in combination.
We then examined the morphological changes and induction of apoptosis in tumor section of U937 xenografts using H&E staining, TUNEL and immunohistochemistry analysis.
