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The HL-60 cells were differentiated by adding 1.25% dimethylsulfoxide (DMSO) to the cell suspension, and differentiation was evaluated by observing morphological changes evident 6 7 days after DMSO addition.
The physiological, behavioral, and morphological changes evident in Arctonotus make it easily distinguishable from any Proserpinus and in combination with the unique changes in P. flavofasciata and P. terlooii inspired this study to investigate the evolutionary relationships between and within the two genera as they pertain to the pace and evolution of novel and unique traits.
Morphological changes, evident using phase-contrast imaging, are apparent upon DMSO exposure and include the formation of membrane extensions resulting in the loss of uniformity of shape.
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Previous applications of unbiased (design-based) stereological principles have expanded our knowledge of neuro-morphological changes evident with the light microscope.
The morphological changes, more evident in the colon cancer cells after contact with both Mn-Zn ferrite and the bio-NCP, are reflected by the sharper distributions.
Contrary to our observations in STS cells, decreases in cell number and morphological changes were evident by microscopy only after high doses of WFA (>2.5 µM).
Moreover, Morgan et al. [19], using horseradish peroxidase, proved that vascular leakage occurred several hours before morphological changes were evident in the brain of mice given an injection of epsilon toxin.
No significant morphological changes were evident under these conditions.
The ripening period is exploited by the germinating spore for molecular reorganization, while no morphological changes are evident (Fig. 1a).
Interestingly, tumor-adjacent urothelia also expressed high levels of CXCL1, suggesting that in urothelial components, early molecular changes occur well before morphological changes become evident.
Although cell surface and nuclear morphological changes were evident upon exposure to DMSO, flow cytometric analysis revealed that PSGL-1 expression was unchanged, irrespective of treatment duration.
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