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CD138 immunostaining was used to objectively evaluate BMPCs because normal plasma cell morphology is not specified and morphological assessment was thought to be too subjective.
Morphological assessment was performed for characteristic features including a lymphoplasmacytic infiltrate, storiform fibrosis, and obliterative phlebitis.
The morphological assessment was performed with May Gruenwald Giemsa-stained cytospin slides.
Morphological assessment was made of tumour extent, obtaining maximum diameter in the axial plane, according to RECIST [ 12], and the corresponding orthogonal diameter.
This morphological assessment was complemented by immunohistochemical staining using a rabbit monoclonal antibody to the Ki67 (catalog no. ab16667; Abcam, PLC, Cambridge, MA) proliferation marker on paraffin-embedded liver sections from the 29 dams.
Whereas H&E morphological assessment was optimized to be independent of stain intensity, stain intensity is the primary readout of IHC quantification and, therefore, is by nature more sensitive to sample preparation.
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The earliest studies using morphological assessment were reported in [10 12].
In these lesions morphological assessment is crucial in order not to delay diagnosis.
Morphological assessment is one important parameter considered in conservation and improvement programs for bovine livestock.
Morphological assessment being a method that relies on visual perception is highly influenced by subjectivity of the observer.
Morphological assessment is only one part of the diagnostic puzzle, but its role should not be underestimated.
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