Lung functional parameters obtained at both time-points were complemented by biochemical and morphological assays, in order to characterize the different phases of the model from a structural and functional perspective.
The effects of DADLE against apoptosis in neurons were measured by the following biochemical and morphological assays: LDH release, RT-PCR, western blot, and TUNEL staining.
In Drosophila, heterozygotes with an additional copy of a CMT2B chromosome are healthy and photoreceptor neurons are not sensitized to stimulation-dependent degeneration within the limits of our functional and morphological assays.
Morphological assays indicated no differences in seed germination rate and seed production between transgenic and WT plants (Figure S2).
The undocking effect of a putative docking factor like syntaxin is expected to be much larger and evident using morphological assays.
Because of their small size, optical transparency zebrafish larvae are well suited for chemical library screens using fluorescent, histochemical or morphological assays [11] [16].
In contrast to the Q128-Htt-induced loss of function of the small LNv, large LNv appear to be spared following expression of Q128-Htt, as shown both by physiological and morphological assays.
In vitro cytotoxicity was evaluated by MTT, apoptosis, and morphological assays.
Morphological assays revealed that overexpression of TaNAC29 delayed bolting and flowering.
Treatment of B103 cells with exogenous Aβ also resulted in a considerable proportion of cell death (40% by the morphological assay and 38% by the MTT assay), which was inhibited by co-transfomation with pcDNA-HA-NIa but not by pcDNA-HA-mNIa co-expression (Fig. 5A and B).
