Combined with previous observations showing that even poor quality blastocysts, when euploid, retain a significant implantation potential (Capalbo et al., 2014a, b), these data further demonstrate the importance of considering all blastocysts for the biopsy and CCS regardless of their morphological appearance, provided that the expanded stage is reached.
We show that the onset of MYC protein overexpression coincides precisely with the morphological transformation of mouse prostate luminal epithelial cells into PIN cells an appearance that is highly similar to human high grade PIN.
Generally, these stripes appear before the morphological appearance of segments, suggesting that some of these genes may play roles in segment formation, although others may only be involved in the ontogenesis of segmental organs rather than segmental patterning.
Based on their morphological appearance, it was proposed that these cells secrete the tectorial membrane [ 12], as during early developmental stages (at birth in mice), the tectorial membrane is in contact with Kölliker's organ through a network of fine filaments, which later detach [ 3, 8, 10].
Additionally, we observed that the morphological appearance of migrating MEF-MYR-CK-B differed from that of MEF-CK-B, and displayed a more flat morphology with broad lamellipodia.
It is likely that the morphological appearance of the PIN cells, which changed precisely at this time of cribriform PIN/CIS development (the cytoplasmic hyperchromasia was reduced and cellular polarity was lost), also reflect an underlying nutrient and/or hypoxic stress.
Boetes found that assessing morphological appearance as well as the rate of enhancement increased specificity to 86%[ 16].
The ability of WRN to affect the growth phenotype in the sgs1 top3 background is supported by our observation that the morphological appearance of large budded cells with undivided nuclei characteristic of S/G2 arrest is restored in the WRN-transformed sgs1 top3 mutant.
The morphological appearance of the regenerating basement membrane that was removed during the procedure, corresponded to the initial phases of its reconstruction: formation of characteristic amorphous layer of extracellular matrix was evident in some contact zones of the epithelial cells with stroma.
In our studies of Lo-MYC and Hi-MYC mice, morphological appearances indicate strongly that only luminal cells, and not basal cells, are positive for high levels of MYC staining and show morphological features of PIN.
