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AFM morphological analysis were executed using an AFM Explorer microscope (Thermo Microscopes, USA) in room temperature, with a non-contact mode through Si cantilevers of a 1650-00 Thermo Microscopesscopes) with a nominal tilt radius of 10 nm and reverberating frequencies of about 220 kHz.
Other specimens used in both molecular and morphological analysis were borrowed from museums (see Acknowledgements).
Flies destined for DNA and morphological analysis were stored in 100% ethanol.
HTCC1062 cells used for morphological analysis were grown in the same medium as for the respiration experiment, in either continuous light (70 µmol photons m−2 s−1) or continuous darkness.
All reagents for morphological analysis were purchased from Bio-Optica (Milano, Italy).
Chromosomal slides for the morphological analysis were prepared as described previously [ 81].
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Morphological analysis was done by TEM.
Morphological analysis was performed on the T1 generation.
Morphological analysis is done using Scanning Electron Microscope (SEM).
In addition, morphological analysis was performed using scanning electron microscopy.
A morphological analysis was carried out using a scanning electron microscope (SEM), (TESCAN, VEGA 3, USA).
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