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Morphological analysis following 24 h in suspension showed that control antibody-treated cells maintained cell-cell contacts and viability (Figure 1Ei); however, whilst neutralising antibody-treated cells exhibited decreased aggregation (Figure 1Eii) this was associated with very low viability.
Apoptosis was determined by morphological analysis following transferase-mediated dUTP nick-end labeling (TUNEL) staining.
DNA was extracted and the cuticles of the ticks were preserved for further morphological analysis following previously published protocols [ 48, 49].
Apoptosis was assessed through flow cytometry analysis of isolated nuclei stained with propidium iodide, carried out on a Becton Dickinson FACS analyser, or by morphological analysis following staining with Hoechst chromatin dye, as previously described by some of us.
For morphological analysis following culture with TDSN, viable (7-AAD negative) Lin-HLA-DR+ cells were sorted (99% purity) using a MoFlo Sorter (DakoCytomation), resuspended in complete medium and cyto-centrifuged.
Our genomewide expression profiling in combination with morphological analysis following JNK inhibition at various phases of EMT revealed that JNK signaling has no functional contribution in the onset of this process.
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Morphological changes in the CP associated with MOG immunization were also examined by quantitative 3-D image analysis following confocal microscopy.
Finally, the images were saved as JPG image format for following morphological analysis.
In addition to MAP-2 staining the neuroprotective effects of PDGF were further corroborated by morphological analysis of dopaminergic neurons following immunocytochemical staining of the cells with TH, a characteristic marker of these neurons.
Morphological analysis of P. falciparum 3D7 following application of the PfSpdSyn inhibitor cyclohexylamine confirmed that parasite development was completely arrested at the early trophozoite stage.
The new image had only two colours, one for cells and another for background, increasing efficiency, accuracy for following cellular morphological analysis.
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