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First, morphological analyses based on T. indicum type and isotypes of T. himalayense, T. sinense and T. pseudoexcavatum specimens showed that Tuber pseudohimalayense and T. pseudoexcavatum have nearly identical peridia, asci and ascospores.
The monophyly of traditional genus Chamaeleo was strongly suggested by morphological analyses based on distinct synapomorphies (e.g., four rotulae in a hemipenis) and it was subsequently supported by a molecular study [ 14].
Although large-scale comparative morphological analyses based on advanced digital imaging modalities have so far been exploited only in a handful of studies [ 10, 14, 28], the predicted increase in digital data necessitates advances primarily in two areas: the remote visualization of complex data and the creation of a universally accepted morphological database.
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Despite considerable disagreement among morphology-based hypotheses, this basic division is supported by nearly all phylogenetic analyses based on morphological data [ 13- 15, 19, 22, 95, 191].
Detailed morphological observations and molecular phylogenetic analyses based on nuclear ribosomal DNA and chloroplast DNA sequences supported the recognition of the new species Begonia jinyunensis.
Although analyses based on morphological and combined data supported this relationship, sequences of the Rbp3 gene alone instead placed Oryzomys among a group that included Nectomys, Sigmodontomys, and a few other genera.
Phylogenetic analyses based on morphological characters have not yielded consistent results either, e.g. [ 9, 10].
The systematic validity of these groups is in full agreement with most recent phylogenetic analyses based on morphological and molecular data [ 26, 28, 34].
Our results confirm that the mitochondrial genome, unlike analyses based on morphological data or nuclear genes, consistently supports the non monophyly of Hexapoda.
However, as indicated in the previous paragraph, the results of cladistic analyses based on morphological data and a larger sample of sabethine taxa casts doubt on these relationships.
Comparative embryological evidence [ 33, 34] and phylogenetic analyses based on morphological [ 28, 35] and some molecular data (EF-1α, EF-2, and RNA polymerase II sequences) [ 36] also suggest that a relationship exists between Diplura and Ectognatha.
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