Our data demonstrate that primary human bronchial epithelial cells (HBECs) are able to undergo EMT in response to transforming growth factor-beta 1 (TGF-β1), as revealed by typical morphological alterations and EMT marker progression at the RNA level by real-time quantitative polymerase chain reaction and, at the protein level, by western blot.
These morphological alterations are known to be typical morphological hallmarks of apoptosis (Häcker, 2000).
For example, SBL-treated cells present typical apoptotic morphological alterations, including karyorrhexis, nuclear condensation and fragmentation, and apoptotic biological changes such as phosphatidylserine (PS) externalization, activation of caspases, and DNA fragmentation.
Likewise, typical evolution of necrotic morphological alterations was observed when the light dose was increased to 3.6 J/cm.
Cells rounded together and were characterized by substantial cell-surface blebbing, typical features of epithelial cancers undergoing morphological alterations associated with apoptosis [ 5].
Therefore, Draq5™ staining additionally served to examine the status of nuclear DNA and to identify morphological alterations of the nuclei, such as those that are typical for cells undergoing programmed cell death.
Thus, sperm with intact and functional membranes present typical coiled tails, contrasting with damaged sperm, were no morphological alterations are detected and straight tails are the norm.
It is unlikely that the effects on amount and localisation of rapsyn-GFP positive structures observed in FLAG-MCLT co-transfected myotubes were due to general morphological alterations, since both FLAG and FLAG-MCLT co-transfected myotubes showed the typical pearl chain arrays of ring-like Golgi apparatuses (compare Fig. 2B C and E F).
Nobody presented morphological alterations at brain CT examination.
In the present study, we showed that exposure to ZnO NPs causes morphological alterations and cytotoxicity.
