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Our study evaluated the antileishmanial effect of AEPa and also morphological alterations indicative of abnormalities in the cell division process.
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Evaluation of nuclear morphology by fluorescence microscopy upon treatment with increasing drug-concentrations revealed Ukrain-triggered morphological alterations that are indicative for apoptotic cell death including chromatin condensation, nuclear shrinkage and fragmentation (Fig. 2a).
The majority of existing techniques to monitor hypertrophy in vitro are based on florescence probes designed to show morphological and biochemical alterations indicative of cardiomyocyte hypertrophy.
Specific labelling of dead cells showed that these morphological alterations are not indicative of atrophic cells.
These data were also supported by phase contrast microscopy analysis of hCMEC/D3 cell cultures (see Figure 1B) showing complete and fully confluent cell monolayers devoid of morphological alterations and/or empty spots indicative of cell injury and death.
Western blot analysis of cells, which were freshly seeded and reacted to DMOG with morphological alterations, did not alter Rac-1 expression as determined by Western blotting (data not shown), indicative of regulation of Rac-1 activity, but not Rac-1 protein.
SEM is a useful tool for studying morphological alterations of platelets that could be indicative of activation and fibrin fiber arrangement.
Recent data indicate that dynamic morphological alterations of mitochondria are involved in mitochondrial dysfunctions [17].
Morphological alterations, including loss of cell-cell contact and elongation of shape, indicative of a more mesenchymal like phenotype were observed with both cell lines when co-cultured with platelets for 24 h (Fig. 2a).
Indeed, we noticed that at both 96 and 120 h AED the silenced discs exhibited morphological alterations in shape, including an evident bending of the A/P boundary that was indicative of localized overgrowth (see Figure 1 and Supplementary Video 1).
Alterations in protein secretion, intracellular signaling, and cell morphology were monitored; and the role of integrin signaling in MSeA-induced morphological alterations was investigated using divalent cation treatments.
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