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The dynamic reconstructions captured in the SWCX design illustrate in this example the arbor-wide structural plasticity across ten time points by highlighting distinct categories of morphological alterations, including elongation, local scaling, retraction, and re-emergences (Fig. 3c).
On a cellular level, neurons exhibit complex disease-related morphological alterations including formation of meganeurites, ectopic dendrites, and axonal spheroids [3].
Indeed, surviving DAn expressing mutated LRRK2G2019S displayed evident morphological alterations, including fewer and shorter neurites compared to untransfected DAn or DAn expressing WT LRRK2 (Fig 4I L and N).
In addition, the overexpression of miR156 in rice, maize, and Brachypodium results in morphological alterations, including increased number of branches, thus providing a favorable phenotype for improved biomass production [ 65, 66].
Morphological alterations, including loss of cell-cell contact and elongation of shape, indicative of a more mesenchymal like phenotype were observed with both cell lines when co-cultured with platelets for 24 h (Fig. 2a).
For example, SBL-treated cells present typical apoptotic morphological alterations, including karyorrhexis, nuclear condensation and fragmentation, and apoptotic biological changes such as phosphatidylserine (PS) externalization, activation of caspases, and DNA fragmentation.
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The most frequent morphological alterations include chromatin condensation, disintegration of the nucleolus, and a reduction in cell volume.
Senescent cells show a series of morphological and physiological alterations including a flat and enlarged morphology, an increase in acidic S-β-gal activity, chromatin condensation, and changes in gene expression pattern.
Infection translated into morphological alterations that included increased granularity (Figure 2A) and was accompanied by a pronounced decrease in both intracellular and extracellular production of NO that importantly, were effectively reversed by Berberine chloride (Figures 2B, C and E).
The morphological alterations were included in a binary database; qualitative variables were transformed into quantitative data by assigning the number "1" if the alteration was present and the number "0" for the absence of morphological alterations.
Indeed, we noticed that at both 96 and 120 h AED the silenced discs exhibited morphological alterations in shape, including an evident bending of the A/P boundary that was indicative of localized overgrowth (see Figure 1 and Supplementary Video 1).
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