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The up regulation of the EMT-specific genes is accompanied by morphological alterations consistent with a loosening of cell adhesion within the aggregates and adoption of a mesenchymal-like phenotype (data not shown).
However, embryos singly injected either with the human NLSmut- or LC8mut-Pak1 mRNA showed very few morphological alterations, consistent with the view that deleterious effects of Pak1 overexpression on zebrafish morphology and survival depend on nuclear import (Figs. 5A and B).
Moreover, with this staining it was possible to observe that cells exposed to cisplatin, in isolation, showed the formation of membrane blebbing, which are morphological alterations consistent with apoptosis.
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MRCP showed morphological alterations in the dorsal pancreas, consistent with chronic pancreatitis and the cross-sectional T1 and T2-weighted images demonstrated atrophy of the pancreatic body and tail.
Consistent with these morphological alterations, cells undergoing EMT show a change in the actin cytoskeleton from cortical F-actin type to stress fiber-like.
Consistent with cell morphological alterations, flow-cytometry analysis suggested that arsenite treatment led to significant cell death in GADD45 β−/− cells, whereas there was no detectable cell death in GADD45 β+/+ cells under the same treatment.
In contrast, the morphological analysis of cardiac muscle tissue revealed pathological alterations consistent with a cardiomyopathy in both genotypes.
Consistent with Pa ExoS-WT ExoS-WT and Pa ExoS-ADPRT ExoS-ADPRTtivity, morphological alterations caused bothhavingwo strains were similar.
Consistent with those previous works, the present study demonstrated that Y-27632 markedly reduced the morphological alterations in the organ-cultured arterial rings.
Nobody presented morphological alterations at brain CT examination.
In the present study, we showed that exposure to ZnO NPs causes morphological alterations and cytotoxicity.
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