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Differential mitochondrial membrane potential decrease, mitochondrial enzymatic dysfunction, and mitochondrial morphological alteration are exhibited in two cell lines.
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This morphological alteration was not apparent after single-agent treatment.
The nuclear morphological alteration was observed by staining cells with Hoechst33342.
The most drastic morphological alteration was observed in the tpm1Δ mutant.
The morphological alteration was observed in control and AgNPs treated HeLa cancer cells.
Many assays for oncogenic transformation have been developed ranging from those in established rodent cell lines where morphological alteration is scored, to those in human cells growing in nude mice where tumor invasiveness is scored.
This morphological alteration is accompanied by an increase in the expression of the protein BNIP3 (Bcl2/adenovirus E1B 19 kDa interacting protein 3) as well as a decrease in the abundance of mitofusins 1 and 2, two proteins involved in mitochondrial fusion.
No morphological alteration was observed in the pulmonary tissue of mice exposed to 10 μmol/kg of (PhTe 2, for 7 or 14 days, when compared with the control group (data not shown).
Dark- or light-grown BMS cells showed no distinctive differences in visual fluorescence intensity or vacuolar morphology (Fig. 8 C, D), suggesting that the observed morphological alterations are either a consequence of the expression of the R and C1 regulators, of the accumulation of anthocyanins or of distinctive properties of the vacuoles in which anthocyanins accumulate.
Thus, sperm with intact and functional membranes present typical coiled tails, contrasting with damaged sperm, were no morphological alterations are detected and straight tails are the norm.
These abnormal chlamydial developmental forms characterized by their ultra-structural morphological alterations are likely aberrant bacteria observed in persistent infections [14], [45].
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