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Specific morphological abnormalities are related to male sub-fertility [ 4].
Several genes listed in Table 1, whose expression is correlated with observed morphological abnormalities, are known to play an important role during organogenesis.
The percentage of patients requiring a surgical intervention is probably even higher if only patients that are suitable candidates for surgery (have morphological abnormalities) are considered.
When lower morpholino concentrations are used, the morphological abnormalities are reduced; however, a bradyarrhythmic phenotype with cardiac conduction abnormalities and a 3 1 or 2 1 AV (atrioventricular) block is observed.
Minor morphological abnormalities are well known to increase in frequency under environmental stress, including exposure to radiation, as is the link between abnormalities and elevated risk of death [ 9].
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Morphological abnormalities were classified into two general categories: head morphology and tail morphology [ 20].
In this study, significant morphological abnormalities were not observed in the zebrafish embryos, when exposed to TiO2-NPs suspensions of different concentrations.
During seed germination, most of the seedlings showed normal two cotyledons whereas small number of seedlings showed tricotyledonous at 20 J/cm2 treatment and no other morphological abnormalities were observed during further growth and development.
As previously reported, no gross brain morphological abnormalities were observed [7].
Despite expression of Maestro in extra-gonadal sites, no other morphological abnormalities were observed despite extensive histological examinations.
Percentages of viability and morphological abnormalities were determined after a vital Eosin stain [20], (eosin 0.5% was prepared by diluting Y eosin in phosphate buffer saline, PBS: 200 ml were obtained diluting a Sigma tablet in pure water. Final concentration: 0.01 M phosphate buffer, 0.027 M KCl, 0.137 M NaCl, pH 7.4).
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