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Fifty yeast morphologic phenotypes were mapped to genetic loci that overlapped with the eQTLs in 19 modules.
Gene signatures corresponding to these morphologic phenotypes were shown to accurately predict clinical outcome in independent datasets of human breast cancers [ 44].
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The morphologic phenotypes can be classified into six categories, each representing an aspect of cellular morphology (Table 2 of [21]).
"Their phenotypes were too different," Matt Wilsey said.
Further, because the analysis is statistics-based, the methodology can be expanded to higher eukaryotes if a morphologic phenotype database is available.
Our shades and phenotypes are not homogeneous.
However unreliable phenotypes are, it is still a persistent construct.
These phenotypes are discussed below.
Our results revealed that deletions of functionally related genes caused similar morphologic phenotypes, enabling loci to be functionally assigned to a specific cellular pathway [8].
We found that QTLs on three chromosomes were shared by morphologic phenotypes and the modules (Table 1).
The current version of our high-content image-profiling system is limited to nonessential genes because morphologic phenotypes of mutants carrying deletions in essential genes are not available.
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