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In this instance, the 10 abdomens were assayed singly, so that the individual abdomen that was SBV-positive could be identified molecularly, to establish exactly which of the 2 species of the C. obsoletus complex was involved and to confirm or refute the morphologic identifications that had been made for the remaining Culicoides species.
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Not only did the molecular results confirm most of the morphologic identifications, but they also showed that C. scoticus seems to have played a more prominent role than C. obsoletus s.s. in transmission of SBV.
Morphologic identifications were made on the basis of published keys (7, 28, 29 ).
These results indicate that morphologic identification has some limitations.
Current monitoring systems are based on a morphologic identification approach, a time-consuming method that may represent a limiting factor for sampling network enhancement.
Such a determination has been made exceedingly difficult given the i) disconnect between morphologically distinct cell subsets and the number of divisions that a cell within a given morphologic classification can undergo [8], [9] and ii) the fact that the amplification that occurs in the bone marrow dilutes the progenitor cells making morphologic identification of these cells unfeasible.
To confirm the morphologic identification of the A. cursor rodent, we compared the mitochondrial control region (fragment of 245 nt) with that of an A. montensis rodent used as a control.
Diagnosis is confirmed by the morphologic identification of the Langerhans cells and positive immunohistochemical staining with CD1a and/or CD207.
Preliminary morphologic identification was performed (by I.B).
Morphologic identification of the species was based on scolex only.
†These segments without scolices were not examined for morphologic identification.
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