In Response: We agree with Eberhard (1 ) that it is difficult to make a species identification when data derived from morphologic examinations do not correlate with those of molecular diagnostics.
During morphologic examinations, normal viable LTEP-a-2 control cells were stained green (0 μg/mL ZnO NPs), and the apoptotic cells exposed to ZnO NPs (0.05, 0.1, and 0.2 μg/mL) appeared as bright green arcs in an early stage and with condensed, yellow/orange nuclei in the late stage).
The kidneys were either embedded in Epon for morphologic examination or snap-frozen in liquid nitrogen and then stored at −70°C until further study.
They should be used in concert with thorough morphologic examination, as part of a rational panel of markers and only in specific circumstances.
In this study, a morphologic examination and a stereologic quantitation of placentas from mothers who smoked cigarettes and who did not smoke cigarettes during pregnancy were performed.
To determine whether the cytotoxicity of DX1 is due to induction of apoptosis, apoptotic cells were determined based on morphologic examination after staining with AO and EB in HL-60 cells.
In the present study, using morphologic examination, we also found that As2O3 (0.5 and 5 ppm) effectively suppressed myogenin expression and retarded muscle regeneration in mouse soleus muscles after injury by glycerol.
QHZ initiated the project and carried out the bioinformatic analysis; QHL carried out animal experiments and determined ID50 of the bacteria on mice; QHZ and HYZ did phage induction, morphologic examination, lysogenization and PFGE experiments; YF was involved in bioinformatic analysis; YGL contributed reagents; RNJ, GRL and SLL coordinated the work; QHZ and SLL produced the manuscript.
Tissue blocks were sectioned at a thickness of 5 μm, transferred to glass slides, and stained with H & E. Morphologic examination of these tissues was evaluated under a light microscope in a blinded manner.
It causes a mild increase in the numbers of cells in long-term liquid culture and a mild block in myeloid maturation that is best appreciated on morphologic examination of Giemsa-stained cells (Tables 1 and 2).
