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These results suggest that the induction of TH1 cytokines during pregnancy by CpG motifs may potentially increase the risk of fetal loss and morphologic defects in mice, at least at high doses, and support the need for further investigation of teratogenesis that may result from exposure to vaccine adjuvants designed to produce TH1 cytokine profile shifts.
At embryonic day 14.5, we observed morphologic defects in 4 out of 5 transgenic embryos examined (i.e., an additional 5 independent lines).
At embryonic day 13.5, we observed morphologic defects in the hearts in 5 of 9 embryos examined (i.e., 9 independent lines).
Morphologic defects in the mutant prostates were evident at P28, and they displayed lower number of ducts with swelling structure compared with those of the controls.
To look for morphologic defects in the TRNs, we stained animals carrying mutations in 13 of the 18 genes with an antibody against MEC-18.
Upon histologic examination, Roy et al. (1998) found apoptosis and altered mitotic figures, along with gross disruption of the architecture of the developing brain, all in the absence of any gross morphologic defects in the other parts of the embryo.
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The remaining 13 genes do not result in gross morphologic defects of the MB.
We reject to make the EndoPAT test in subject with morphologic defects or finger wounds.
Likewise, mutations in ref-1, hlh-29, and hlh-25 cause morphologic defects that affect sexual reproduction.
Recent literature suggests, however, that a shift toward a TH1 cytokine profile during pregnancy may increase the risk of fetal morphologic defects.
As early over-expression of Ras-Raf-Mek signaling causes morphologic defects, we are currently generating transgenic lines that allow temporal control of Mek signaling, which will be a valuable tool for deeper genetic dissection of the Int6-Mek-Erk relationship in vivo.
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