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In contrast, other organotins were weaker cell death inducers and generated mild morphologic changes (data not shown).
Morpholino knockdown of exon 9 in col6a3 caused a severe phenotype with both significant motor deficiencies and widespread morphologic changes (data not shown).
Importantly, cell death was not observed before 2 hr treatment with TPT, as shown by lack of caspase-3/7 activation, PARP cleavage, and cellular morphologic changes (data not shown).
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Of note, 84.6% of embryos injected with half concentration of exon 9 morpholino exhibited the same phenotypic (both motor and morphologic) changes (n = 78, data not shown).
There are very few data linking early morphologic changes to long-term clinical outcome.
We acknowledge that a limitation of our analysis is the lack of in vivo functional correlation to the morphologic changes observed in the in vitro data presented.
The morphologic changes correlated well with the gross observation data.
Due to an absence of empirical data of the detailed morphologic changes and other changes that occur during progression of in situ cancers, such theories may be difficult to evaluate.
This is accompanied by enlargement and morphologic changes referred to as 'fried egg morphology'.
We have found that TCDD causes morphologic changes in cultured mouse embryonic salivary glands (Kiukkonen A, et al., unpublished data).
The increase in the number of hypopliod nuclei and the observation of some morphologic changes as vacuole formation seems to relate NOC-12 with autophagy (personal data).
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