In the present study, we examined the morphological and cytochemical effects of agaritine on U937 cells to elucidate the tumoricidal mechanism of agaritine.
This review will focus on some morphological and cytochemical techniques used to demonstrate the presence of apoptotic activity in tissue and cultured cells.
Diagnosis was established according to standard morphologic, cytochemical and immunophenotypic criteria and all patients were enrolled in successive multicenter studies of the "Programa para el estudio y tratamiento de las hemopatias malignas" (PETHEMA) Spanish study group.
In 45 previously untreated patients with de novo AML, peripheral blood and/or bone marrow samples from all patients were subjected to microscopic morphologic examination, cytochemical analysis, immunophenotyping and karyotyping.
Diagnoses were carried out on the basis of clinical features, blood counts, peripheral blood films and bone marrow morphology including cytochemical staining and immunocytochemistry where available.
The diagnosis of ALL was based on morphologic, cytochemical, and immunophenotypic criteria.
Diagnosis was established according to standard morphologic, cytochemical and cytogenetic criteria.
There is a strong need to bring together the biochemical, molecular biological, genetic, morphologic, immuno-cytochemical, and fine structural results.
The morphology of the recorded cells was revealed by injection and cytochemical visualization of the biotinylated tracer Neurobiotin, as described elsewhere [6], [14], [16], [17].
To further corroborate our findings, we investigated the expression of osteogenic markers using immunohistochemistry and cytochemical staining.
