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Morphologic analyses were carried out by only one investigator in a blinded manner; hence inter or intra-individual variability was not assessed.
Morphologic analyses were carried out on formalin-fixed, paraffin-embedded sections of tumor xenografts were cut at 5 μm and allowed to air dry.
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For morphologic analyses, mice were serially killed at 1 2, 3 4, 6 7, 14 and 21 months of age, a minimum of five mice in each group.
Morphometric and meristic analyses were also done to assess morphologic variation.
Morphologic evaluation, immunohistochemistry, in situ hybridization and enzyme immunoassay (EIA) analyses were applied.
Differential scanning calorimetry and thermogravimetric analyses were carried out together with Fourier transform infrared spectroscopy (FTIR) and morphologic characterization of the samples.
Both analyses are flawed.
Morphologic analyses revealed that syncytiotrophoblasts were significantly less abundant in differentiated PPARγ−/− than in PPARγ+/+ TS cells (4.7±0.7% vs. 7.3±1.0%, respectively, P = 0.023) (Figure 4).
Rodent species were identified on the basis of morphologic analyses of formalin-fixed carcasses; chromosomal data and mitochondrial DNA sequencing of the cytochrome b gene were used to confirm identification of most antibody-positive animals.
For sparse labeling of neurons without selection for a particular subtype, the R26CreER Z/AP combination with an early postnatal injection of 4HT generates a frequency of labeling that is close to optimal for morphologic analyses [ 0.001% 0.01% of neurons labeled; 5,10].
In morphologic analyses we found that a majority of the mass was fibrous and composed of many types of fibers (e.g., mineral wool, fiberglass, asbestos, wood, paper, and cotton).
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