Sections of the MT-stained tissue were analyzed in four cross-sectional defined areas for arrangement and collagen content histologically and quantitatively by using Meta-Morph analysis (Molecular Devices).
Density of CGRP-IR in specific regions of the spinal cord was determined with the aid of Meta Morph image analysis software (version 4.5r4; Universal Imaging, Downingtown, PA) as previously described [ 17, 18].
For species with heteromorphic sperm we used the length of the longer sperm morph in the analysis (thereby keeping the comparison within the fertilization-competent sperm morph, [ 31, 32]).
Finally, the performance of the proposed filter bank-based VC model is compared with the state-of-the-art multiscale voice morphing using RBF analysis.
Following the results of the LC-MS/MS analysis, bulgy morph and non-bulgy tadpoles were subjected to immunohistochemical staining for histone H3 and 14-3-3 14-3-3 14-3-3
Lastly, although blue males gained some matings as a result of aggressive interactions and variable female mating preferences [ 50], the factors that allow for the persistence of this morph is under current analysis.
From one to five specimens of each morph were used for the analysis.
For area measurements, brain regions were video-digitised on a PC equipped with an image analysis system (Meta Morph, Visitron, Germany) and measured by the built-in measurement tools.
For the forewing analysis this involved Morph, Stage, Wing Section, and Morph*Stage*Wing Section as fixed effects and Slide as a random effect.
All images were recorded using a computer-assisted image analysis program (Meta Morph) according to the manufacturer's protocol.
To further explore patterns of differential expression in relation to both feather and morph, we conducted a qPCR analysis of seven genes selected from Table 4.
