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My dad, who was in the navy, would make dinner in the morning, plate up a meal for each of us and put it under clingfilm with our name on top.
The following morning, plate pairs with about 100 colonies formed were used to calculate the stability ratio of the plasmid.
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While Roland became a national sensation with millions tuning in every morning, plate-eyed in wonderment (it was the 80s), Kevin seemed destined to be forever the bridesmaid.
In the morning, plates were screened for blue colonies indicative of phoN::TnphoA alkaline phosphatase activity and PhoQ activation by divalent cation limitation.
One afternoon plate of burrata, Puglia-style cream-stuffed mozzarella from the local cheesemaker Gioia, is dressed up with strawberries, arugula, blossoms and toasted bread, like a Nordic take on a Caprese salad.
The next morning, the plate was washed 3 times with PBS and blocked for 2 hours at room temperature with 200 µl blocking buffer (3% BSA (Probumin; Millipore) in PBS).
On the next morning, the plates were centrifuged 3,000rpm for 10 minutes at 4°C, the PVDF filter plate was discarded and the receiving plate (containing the eluted protein) was sealed and frozen at −80°C.
Every piddly Monday afternoon selling plate can be observed somewhere amid the vast emptiness of the television galaxy.
At 3 on a recent morning, huge plate-glass windows offered a blinding view of the store's blazingly lit white interior.
Mr. Ramirez has an enormous collection of china, and over 20 courses one evening, no plate was used more than once.
(7) The plate is then read in a Bio-Rad xMark Microplate Spectrophotometer (any standard absorbance reader with the appropriate filter is sufficient) at an absorbance of 405 nm.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com