Moreover, passaging may result in a selection pressure for parts of the cell population, for example adherent cells vs. trypsin-sensitive cells, that are being removed to a different extent from the culture flask during passaging and that may alter the overall gene expression profile in higher passages and lead to different results when compared with earlier passages.
Moreover, early passages (passage 39 61) and late passages (passage 75 124) of two HPV16 and two HPV18 immortalised cell lines (FK16A, FK16B, FK18A and FK18B) were included, as well as anchorage-independent clones of HPV16 (FK16ASA)- and HPV18 (FK18BSA -containing cell lines.
Moreover, its passage, while metronomically regular, is lethargically slow, and the principles of diffusion that control the process are largely impervious to interference.
Moreover, blood passage through the ECMO circuit did not seem to generate more procoagulant, endothelial, leucocyte-derived or platelet-derived MPs, compared to baseline.
Moreover, the passage of large molecules through the channel interrupt the ion current causing fluctuation or even transient blockages of conductance [17], [23], [25].
Moreover, consecutive passages showed accumulation of chromosomal alterations overlapping with those found in cervical (pre)malignant lesions (Steenbergen et al, 1998a, 1998b, 2005; Wilting et al, 2006) and shared altered expression of certain genes with these (pre cancer stages (Steenbergen et al, 1996, 2002; van Duin et al, 2003).
Moreover, with the passage of time, The Producers became a cult favourite and was eventually widely lauded as one of the greatest comedies ever made.
Moreover, despite the passage of UN Security Council Resolution 1701, which calls for Israel's withdrawal and the disarmament of Hizbullah, neither of those aims is likely to be achieved soon.
Moreover, with the passage of time, particles were seen to fuse together, forming structures that resemble dividing bacteria.
