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Significantly more vesicular staining was observed in cells expressing PAM/Endo (Fig. 2d).
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Liposomes are spherical-shaped vesicles comprised of one or more vesicular bilayers (lamellae) [96].
Grey, relatively less vesicular pumice clasts with higher microlite contents displayed a BSD with an equivalent vesicle diameter mode of ~15 μm, while the more vesicular, less crystalline white pumice clasts displayed an equivalent vesicle diameter mode of ~50 μm.
ORF8b and ORF14 showed a vesicular staining, and nsp2 was found both in cytoplasm as well as in the nucleus.
No vesicular staining remained visible, which supports the hypothesis of a role for the AdcA C-terminal domain in endosomal targeting.
YFP-Nedd4 was transiently transfected and the Nedd4 protein was found localized to the perinuclear region, the plasma membrane, and also exhibited some cytoplasmic vesicular staining (Figure 6).
The SNAP25 antibody detected luminal cells, with a vesicular staining pattern.
The dye shows diffuse staining in non-autophagic cells, but is sequestered into punctate vesicular staining when autophagy is induced.
Vesicular staining of PAM/Endo was not coincident with staining for LAMP1 (Fig. 2e) or ACTH (not shown).
In DIV1 2 neurons, an intense vesicle-like pericentrosomal BICDR-1 staining was observed, whereas a weak vesicular staining was present in some processes.
PAM/TGN also accumulated in puncta surrounding the nucleus, overlapping compartments containing giantin (Fig. 4c, d); very little dispersed vesicular staining was observed.
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