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It might also be the case that younger Min speakers nowadays indeed adopted more variants in /dz/ realization, possibly due to their lower frequency of use and lower proficiency in Min (Fig. 2).
Two or more variants in the same patient might also have a role in explaining the diversity of clinical phenotypes.
It will be important to – in the future – include many more variants in this type of analysis, to look for subgroups within patients.
To investigate whether individuals with combinations of TIRAP SNPs might influence disease occurrence, we undertook analysis of the frequency of combinations of two or more variants in cases and controls.
NGS technologies have opened up great opportunities for discovering more variants in the human genome.
Additionally, GWAS consider multiple variants in each single gene and even more variants in non-coding regions.
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There were more variants located in telomeric regions than in near centromere regions, which was in accordance with the distribution patterns of genes in maize [ 30].
For genes in which two or more variants (not in perfect LD) were associated at p<0.01 with a given quantitative trait, we also performed haplotype analysis to discern which allelic haplotype might be contributing to variation in the trait.
The sequences generated by the RainDance enrichment contained several more variants, 221 in total, including 26 not identified in the SureSelect-enriched sequences.
We found many more variants with annotations in interesting categories when using ENSEMBL transcripts compared with REFSEQ transcripts only.
As expected, we observed more variation (especially more uncommon/rare variants) in ABs.
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