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The control region had high variability but only 1.5 times more variable sites and 1.6 times higher nucleotide diversity per site (Pi) compared to the ATPase region (Table 1).
Without correction, more variable sites would have a wider distribution of Res values and thus an increased chance to randomly surpass selection threshold.
The ascertained loci revealed significantly more variable sites (described in Text S1) and more haplotype diversity (ke3, P<0.05) than the typing loci (Table 1).
However, because these regions encompass 80% of the total sequence length, proportionately more variable sites occur in the coding regions (22%; 21% for third codon positions) than in the non-coding regions (14%).
Two or more variable sites showing an identical pattern of compatibility are considered to form a recombination block [ 50].
Comparing with 12S rRNA genes, 16S rRNA genes of asteroid species seem to have more variable sites.
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With the inclusion of more variables, site separation became more complete.
The ACID algorithms were primarily aimed at annotating attC sites in CI and some of the more variable attC sites found in cassettes in MRI are currently missed.
Mean local environmental conditions also affected trait variation; in most of the species both vegetative and reproductive traits were more variable in sites with higher nutrient contents and higher light availability.
In contrast, the large spread of crayfish PAH scores indicates that crayfish PAH profiles were more variable between sites.
The two temperature estimates are less similar to eachother; mean April temperatures (ca. 0 6°C) tended to decrease towards the North, whereas mean spawning-time temperatures (ca. 2 10°C) were more variable between sites.
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