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NG-MAST allele numbers of the more variable segments of porB and tbpB, and sequence types (STs) were assigned using the NG-MAST website (www.ng-mast.net).net
Amplification and sequencing of the more variable segments of the porB and tbpB genes examined in NG-MAST [ 44, 45] was performed as previously described [ 46].
NG-MAST allele numbers of the more variable segments of porB and tbpB, and sequence types (STs) were assigned using the NG-MAST website (http://www.ng-mast.net).net
The more variable segments of porB (490 bp) and tbpB gene (390 bp) examined in NG-MAST [ 40] were sequenced as described previously [ 48].
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Thus far, such exon-focused methods (e.g. EPIC) have been exploited in most of the SNP discovery surveys, and no study exists where the more variable, non-coding segments of the genes (e.g. introns) have been utilized to design specific primers aimed at binding to only one of the duplicated loci.
This window size was chosen because it was intermediate between the average marker distance (approximately 25 kb in the GBS datasets and 1.5 kb in the HDRA datasets) and the distance at which LD decayed to half its initial level (estimated at 100 kb for the indica panel and 150 kb for the japonica panel on average across all chromosomes but much more variable in short-range segments).
Rate of recruitment at vents is more variable among studies within a segment than among segments.
Interestingly, the three TLRs with more PSC in variable segment of LRRs are the viral TLR7, 8 and 9, with 71.43%, 84.0% and 75.0%, respectively.
Long-term dynamics of indicators within the biodiversity segment were more variable and changes in the overall biodiversity status were therefore less conclusive.
In a separate sequence (not shown), instead segment u maintained a relatively constant velocity (1.55 ± 0.09 mm/s), while the surrounding segments were far more variable (1.51 ± 0.48 and 1.23 ± 0.32 mm/s for j and a).
The basic approach involves the use of universally conserved polymerase chain reaction (PCR) primers to amplify segments containing the more variable regions, which are then sequenced through technologies that have become increasingly advanced over the past decades (Bartram et al. 2011; Caporaso et al. 2011, 2012).
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