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Why? Did ideologically dedicated conservatives prefer Pence's consistently conservative track record to Trump's more variable positions?
Two or more variable positions in a protein may coadapt to conserve interactions needed for proper structure or function [1] [3].
While the outbred Wistar strain contains much more variable positions, lack of detectable polymorphisms in a few animals can obscure a potential informative marker.
Conversely, more variable positions are located in the region encoded by exon 6, exon 7, and for a large part in exon 13 (Additional file 2).
In derivative molecules, more and more variable positions were replaced by degenerate bases, starting either from the 5′-end or 3′-end (Table 2).
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The inner antisense primer was repositioned to a more variable position in the VP4 gene between species A, B and C sequences to enable selective hybridisation and strand extension of HRV-C sequences.
While this water molecule occupies a much more variable position in the cooperative group (BcII, IMP-1, and VIM-2, Figure 7A), it is present in a much higher fraction of the structures, suggesting these enzymes are better poised to turn over the intermediate, once it is formed.
The high nucleotide conservation for EVADR corresponds to an evolutionarily conserved secondary structure in OWMs and apes, with significantly more variable nucleotide positions occurring in unpaired or loop positions (47/152) than in structured regions (39/242 of paired nucleotide positions) (P = 0.0008; Chi-squared test; Figures S7 and S8 in Additional file 1).
Nevertheless, RRT results showed that conserved codon positions also contribute significantly to neobatrachian-specific rate acceleration, displaying similar trends to those experienced by more variable codon positions (see Additional file 3).
The striking difference in percentage estimates between nucleotide and amino acid levels could be explained by saturation of more variable codon positions in the former.
The sense primer for GII (NV107a) was supplemented with an oligonucleotide primer containing inosin at the more variable nucleotide positions (NV107c).
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