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As an example, genes expressed only in a particular tissue lineage display more variable expression than the genes expressed systemically (compare Figure 1 and 3).
Another HSPC member, Hspc5, exhibits a more variable expression pattern, but is generally highly expressed.
For variability between tissues, we found that more variable expression was associated with longer intergenic sequences [4] [6] (Figure 4A, B), and with shorter genic sequences (Figure 4C, D).
For level we used the median across-tissue expression; for the expression pattern we used the variability of the expression across tissues (a more variable expression is likely to represent a more tissue-specific expression pattern); and for noise we used the variability between biological replicates, i.e. between groups of plants grown independently (see Methods for precise definitions).
While nasopharyngeal carcinoma and T-cell lymphomas frequently express LMP-1, the tumours show more variable expression of the protein and therefore might be less susceptible to statins.
Oct4 and Sox2 showed relatively uniform expression across all populations, while epiblast markers shown to be heterogeneously expressed, such as Nanog and Stella, exhibited more variable expression.
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MHCII expression is more variable than expression of MHC I, both within an individual immune system and at the population level, and most cancers and normal tissues do not express MHCII.
Studying the triangular relationship between methylation, gene expression, and GC3 (figs. 2 and 3), we observe that GC3-rich genes tend to have more variable gene expression and lesser gene-body methylation levels than the GC3-poor genes.
Species- and sex-specific patterns were observed in stress response and recovery, with pink salmon mounting a higher magnitude stress response for plasma variables and sockeye salmon exhibiting a higher and more variable gene expression profile.
Therefore, the consequences of switching on an upstream activator include higher average expression, and more variable yet coordinated expression across samples, which matches our observations (Tables 5 and 6).
More, despite variable expressions between tumors, analysis of both quantified mRNA with real-time PCR (RT-PCR) and protein levels with Western blot show a close correlation between these two regulation levels.
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