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PCAs of combined datasets can artificially force the major axes of shape variation present in the more variable datasets through the data derived from the less variable dataset or sets.
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Here, we undertook to examine the relationship between flake size and efficiency parameters using a larger and more variable flake dataset than used in previous analyses.
Conversely, smooth areas have more variable registration across datasets, and the attendant misalignment leads to poorer performance when comparing predicted with observed effect sizes.
As some of the sequences got very short (datasets def; Additional file 2, Table S2), we produced additional subsets using a value of 0.5, retaining more variable sites and fewer sequences (datasets seq).
A clear pattern in reduction of RMSPE can be seen by including more diverse combinations of predictor variable datasets (Table 5).
The Taguchi method was effective at identifying successful architecture with the potential for development as a useful generic inhaler ANN model, although this would require much larger datasets and more variable inputs.
Moreover, the nrDNA dataset contained at least ten-fold more variable sites than the full cpDNA dataset (see Additional file 3: Table S3), suggesting that the little variability in the latter (<2.0%) and associated homoplasy might be responsible for the artifactual positions of some species and lack of resolution at the base of the tree.
Here, Do et al., first report that replicates from the Letourneau et al. (2014) dataset are more variable than one would expect as determined by principle component analysis (PCA).
For some datasets, goodness-of-fit was more variable, e.g. the German pertussis PT IgG dataset.
Therefore we performed all other analyses with the complete datasets, including the more variable regions.
The situation may be even worse in ecology, where datasets are typically much more variable and few dedicated repositories exist.
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