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The investment is of a piece with Natauri's broader thesis that healthcare investment activity broadly is about controlling the engagement with patients earlier and more upstream in the process.
Extended interdisciplinary is promoted by the European Commission in terms of 'responsible research and innovation', with the aim to integrate and align societal needs and demands more upstream in the technological development process (Von Schomberg [2012] and Von Schomberg [2013]; Owen et al. [2012]).
The fact that laminins are more upstream in the signalling cascade than dystrophin could explain why we observed a downregulation of Kir4.1 and aquaporin-4 protein expression in β2−/−/γ3−/− instead of a mere redistribution and/or disturbances in membrane insertion.
The problem, therefore, lies more upstream, in an earlier stage of biomolecular grounding (learning through perception [icons] and conceptual instructions).
In particular, the more upstream in a pathway the temporary change occurs, the stronger the transcriptomic entropy increase.
The direction of an edge would be from the stem whose 5′ strand is more upstream in the sequence.
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The reading frame overlap and the absence of a poly(A) tail from the more upstream gene in each pair (atp8, nad4l) most likely mean that in each case the two genes are expressed from the same, bicistronic transcript (atp8/ and6 anad4l/4l/ nad4l/
Nodes with higher estimated relay ability are located at more upstream positions in the trees, while nodes with lower estimated relay ability are positioned downstream to avoid the propagation of negative effects.
It is therefore important to try to target the more upstream events in the cellular cascades leading to the disease.
Moving the ntla MO targeting sequence and the S-photo-MO to a region more upstream resulted in phenotypes specific to ntla function.
This explains the absence of poly(A) tails downstream of the ORF of the more upstream gene in each case (atp8 and nad4l; Fig. 4).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com