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Co-cultivation for 60 hours yielded more transformants than at 48 hours; however, more transformants with growth defects tended to emerge on the SM plates.

We observed more transformants for the rARS-containing plasmid in the eco1 background compared to WT, using the same amount of plasmid DNA (Fig C), suggesting more firing of this ARS in the mutant, consistent with the BrdU labeling experiment.

In contrast, progeny of ndc80 Δ/+ ipl1 -2/+ transformants with pRS316: ndc80 -8 include more than 2 viable spore clones per tetrad.

In tomato we observed that complementation of SlMLO1 loss-of-function mutants with CsaMLO8 restored PM susceptibility, with individual transformants with higher CsaMLO8 expression generally being more susceptible to PM than transformants with lower CsaMLO8 expression (Fig.  2).

In our study, one more renewal of the regeneration medium was done after selecting the transformants with 10 mg/l hygromycin, and the concentration of hygromycin was increased to 15 mg/l.

Transformation of the alb1 mutant with pAUR-PksP yielded transformants with black conidia, whereas all transformants of alb1 with pAUR325 remained albino (Figure 3B).

Importantly, we found that glucoamylase production levels were much more consistent among the transformants that harboured the expression cassette at the targeted locus as compared to transformants with a random integration pattern.

(B) Pigmented colonies formed in transformants of alb1 with pAUR-pksP (left), but transformants with pAUR325 remained albino (right).

Transformants with elevated laccase production might be generated by modifying the pGL promoter region and/or by screening among a larger number of co-transformants.

The size of the nanobody library is 8 × 108 transformants with great diversity.

Transformants with DNA inserts on the second, third and X-chromosome were obtained.