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That could help explain the promiscuous effects of many siRNA and shRNA inhibitors; a seven-base sequence can potentially bind many more transcripts than a 22-base sequence.
However, Hv larvae regulated more transcripts than did Ms larvae and regulated transcripts not regulated by Ms larvae (Figure S1, upper and lower panels).
Hence, when feeding on the same defenseless plants, Hv larvae regulated 16 times more transcripts than did Ms larvae (Figure 1B).
The regulation was more pronounced in Ms larvae which regulated 1.9 times more transcripts than did Hv larvae in response to the overall JA-mediated defenses excluding N and N/PI (Figure 3A, inset, upper and lower panels).
For example, for serine peptidases, Ms larvae that fed on JA-silenced plants regulated 1.5 3 times more transcripts than larvae than that fed on N/PI- and N- silenced plants, respectively.
Here we demonstrate that the E. coli transcriptome consists of far more transcripts than the present annotation suggests, of which many transcripts seem important to the bacterial stress response.
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Some white flowered lines accumulated more transcript than colored flower lines, suggesting that other factors in the background may be more important than nonsense-mediated decay.
The filtering step left only one candidate gene, At5g61420, that co-expressed with biosynthetic genes for aliphatic glucosinolates and that had a cis-eQTL which led to the Sha allele having approximately 3-fold more transcript than Bay.
For two genes (GRMZM2G140537 and GRMZM2G104760), the transgenic plant showed more transcript than did the non-transgenic plant; this increase in abundance was 2.5 and 3.2 fold, respectively.
As expected, the GY line had the same or even more transcript than the wt for all three genes, while the GAY line had significantly lower expression levels, similar to bzip63.
This work represents more than 100 times more transcript sequences than the total previously submitted to GenBank.
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