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As expected, genes with more transcripts lead to more difficult estimation problems and decreased performances for all methods.
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Transcriptome profiling with a microarray representing more than 81% of known Ae. aegypti transcripts lead to the detection of 3512 transcripts of which 70 were differentially transcribed in the LiTOX strain.
For instance, the larger number of replicates used in our study, our RNA amplification strategy and microarray analysis of more transcripts has probably lead to the identification of almost twice the number of mRNA targets compared to Morris et al. (1424 vs. 726) - most of them (>80%) bearing a PUM motif in the 3'-UTR or coding sequence.
A similar mutation was shown to create a more efficient mRNA cleavage site in the pro-thrombin gene where more efficient processing of the transcript leads to higher pro-thrombin levels and a higher risk of thrombosis [50].
Our data also show that the transcript accumulation in the somatic cells is more sensitive to blue light (14 genes), while red light led to accumulate more transcripts (15 genes) in the reproductive cells.
Conversely, in P. trichocarpa, higher abundance of ARF1 transcript leads to a more abundant supply of ARF1 protein, which increases the relative rate of endocytosis and PIN polarization.
Secondly, any probe that binds two or more transcripts will be averaging across transcripts and likely lead to decreased estimates of variance.
VPA treatment could, for example, lead to de-regulation of one or more transcripts encoding polypeptides responsible for the observed effects.
More transcripts, more letters, more recommendations.
Suhr also announced the release of more transcripts of text messages to the Associated Press.
For a protein species nomenclature we need to distinguish various levels: At the gene level a gene may have one or more than one transcript (transcript level); each transcript may lead to one or more than one translation.
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