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Furthermore, about 30% more transcripts have been found in the SBS library than in the MPSS library.
In particular, about twice more transcripts have been found in the hypocotyl for this gene compared to Gmpgip5, confirming the higher level of expression of Gmpgip3 shown by the qRT-PCR results (Table 1).
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However, in the MII-D2 transition, more transcripts had decreasing expression than increasing expression (Table S1).
Transcriptional starts only considered unique start sites; i.e., if two or more transcripts had a common start site, the site was only counted once.
For instance, the larger number of replicates used in our study, our RNA amplification strategy and microarray analysis of more transcripts has probably lead to the identification of almost twice the number of mRNA targets compared to Morris et al. (1424 vs. 726) - most of them (>80%) bearing a PUM motif in the 3'-UTR or coding sequence.
In recent years, many more lncRNA transcripts have been identified.
A recent global analysis of mRNA stability in M. tuberculosis concluded that more abundant transcripts have shorter half-lives (Rustad et al., 2013).
Although more than 14,700 genes and more than 16,100 transcripts have now been predicted, the functions of approximately 40% of the gene products remain unknown and in silico annotations of many others still require verification [ 1, 2].
Only those GOs accounting for more than 10%% transcripts have been plotted in the graph.
Of these, more than 75 transcripts have roles in mitochondrial regulation and degradation.
One complication that we faced was that the CV of a transcript level among animals depends on the expression level of a transcript, with more abundant transcripts having less variation – as would be expected, since more abundant transcripts could be measured more reliably than the less abundant ones.
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