There were more total lipids that were down-regulated by 2,4DNT, 2,6DNT, 2ADNT and TNT except 4ADNT (Figure 10B).
In this relation, it could be concluded that the resistant mutant of bacteria to antibiotics had more total lipids and phospholipids contents than sensitive one [ 10, 11].
Our results also agree with Ryckebosch et al.'s earlier work where different solvent systems were compared on Chlorella, and the authors showed that a 1 1 ratio of chloroform methanol recovered significantly more total lipids than a 2 1 ration of chloroform methanol.
A characteristic of these organelles is their unique lipid composition: unlike LDs found in cells of other tissues, HSC LDs comprise equal amounts of REs and TGs accounting together for more than 75% of total lipid content [96,102 105].
De novo fatty acid synthesis by tumour cells accounted for more than 93% of total lipid fatty acids in an experimental tumour model, indicating that endogenous fatty acid synthesis could be a significant source of fatty acids for tumour cell growth and survival (Ookhtens et al, 1984).
The accumulation of fat in the liver is pathogenic if total lipid exceeds more than 5% of liver weight or hepatocytes containing intracellular fat droplets are above 5% [ 1].
It produced EPA at about 50 % FAME but with a total lipid of more than 50 % DCW; therefore, strain Z5567 produced EPA at about 25%% DCW, which is about 25 and 67%% improvement over strain Z1978 and Y4305, respectively.
This result also confirmed that oyster shells can be used for the growth of Chlorella sp. as well as increasing the total lipid content more efficiently without an additional supply of carbon, although a higher lipid content was observed in the control group.
Though batch fermentation was found to be optimal for growth and total lipid production, ARA was synthesized more rapidly in fed-batch cultures.
In shake flask and micro-bioreactor experiments, dry cell weight (DCW) is also determined due to the availability of more sample volume (~5 mL), so that the total lipid content of each sample (total lipid/DCW) is determined to better evaluate the biomass, lipid, and EPA productivities.
