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For specimens histologically non-homogeneous we extracted more tissue cores, representative of the different areas.
Briefly, we obtained two or more tissue cores from each specimen that was 1.8 mm in diameter and 1.0 3.0 mm in length depending on the tissue depth in the donor block.
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All cells of each tissue core included in the TMAs were evaluated, and only cases containing two or more preserved tissue cores were scored.
More than two tissue cores were extracted to minimize extraction bias.
Only tissue cores with more than 25% of tumor cells were analyzed.
241 cases were contained in previously well-characterized tissue microarrays (TMA) and only tissue cores containing more than 95%% tumor were included in this analysis [ 5, 19, 21– 23].
In the situation of observing different staining intensities between the duplicate tissue cores, the core with more representative tumor tissue was chosen.
Only tissue cores exhibiting one or more shed cells were scored.
Third, organoids create more tissue for testing.
"With more tissue you have more to learn". he said.
These controls provided positive staining in all tissue cores.
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